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KMID : 1161520170210060388
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Animal Cells and Systems
2017 Volume.21 No. 6 p.388 ~ p.396
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B16 melanoma expressing EGFP as a self antigen is differentially immunoedited by tolerogenic thymic epithelial and dendritic cells
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Kim Su-Gang
Kim Ki-Yeon
Hong Seok-Mann
Kim Moon-Gyo
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Abstract
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To investigate how the immune system responds to tumor self antigens, we used enhanced green fluorescent protein (EGFP) in B16 melanoma cells (B16-EGFP) and tested in the mouse lines expressing EGFP in thymic epithelial cells (3.1T-EGFP) or in antigen presenting cells (Get40), in comparison to the wild-type mouse. B16-EGFP cells were distinctively immunoedited in three mouse lines at the early phase, and the cells were completely eliminated only in the wild-type at the late phase, suggesting EGFP-specific tolerance is present in 3.1T-EGFP and Get40. The numbers of tumor-infiltrating T cells in all mouse lines were reversely correlated with the tumor sizes, suggesting dominant T cell mediated tumor elimination. When a soluble EGFP was immunized, surprisingly, the growth of B16-EGFP in Get40 mouse was promoted, while reduced in B6. Immunization did not make significant difference in the growth of tumors in 3.1T-EGFP. Detailed analyses showed the opposite directional changes in the numbers of B and CD8+T cells in B6 and Get40. In Get40 mice, the immunization significantly reduced the percentage of Gr1?CD11b+ cells, indicating that tolerance induction and breaking involve both adaptive and innate cells differentially. Therefore, the strategy for a cancer vaccine should be carefully considered on the types of antigen expressing cell.
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KEYWORD
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Immunological tolerance, EGFP as a tumor self antigen, immunoediting of cancer
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